Ointment:Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods.
Article Snippet: .. Materials Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019-360-60) O2 gas Eye ointment (Bausch & Lomb, 3-10119-02239-9) Dexamethasone (Bimeda, 1DEX022) 10% povidone-iodine antiseptic (Betadine, 67618-150-17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006-12) Graefe forceps (Fine Science Tools, 11053-10) Heavy blade dissector scissors (Fine Science Tools, 14082-09) Kelly hemostats (Fine Science Tools, 13019-14) Needle holder and scissors (Novo Surgical, G2171-25) Dissecting jewelers microforceps (Fisher Scientific, 08-953F) Periosteal elevators (Roboz, RS-8820) Surgical scalpel, #3 (Fine Science Tools, 10003-12) Cold sterilizing tray (US Dental Depot, 208GST-2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA-Advantage, 3-948) Anesthesia induction chamber (SOMNI, IC-3014) Oxygen concentrator (ProO2, Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non-rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP-1M) Temperature control unit (Kopf Instruments, TCAT-2LV) Waste gas catch (SOMNI, WG-15005) Dissecting microscope (Zeiss, Stemi 2000-CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806-WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High-temperature, fine-tip cautery (Bovie, AA01)Beacher et al. 4 of 21 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70090 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Stainless steel screws (McMaster-Carr, Phillips, no. 0, 1/8-in. length, 92470A015) Syringe with 27-G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/ miniscope_v2.0/ tree/main/STL-miniscope) and baseplate (Base_R007.
Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods
Article Snippet: .. Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019‐360‐60) O 2 gas Eye ointment (Bausch & Lomb, 3‐10119‐02239‐9) Dexamethasone (Bimeda, 1DEX022) 10% povidone‐iodine antiseptic (Betadine, 67618‐150‐17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006‐12) Graefe forceps (Fine Science Tools, 11053‐10) Heavy blade dissector scissors (Fine Science Tools, 14082‐09) Kelly hemostats (Fine Science Tools, 13019‐14) Needle holder and scissors (Novo Surgical, G2171‐25) Dissecting jewelers microforceps (Fisher Scientific, 08‐953F) Periosteal elevators (Roboz, RS‐8820) Surgical scalpel, #3 (Fine Science Tools, 10003‐12) Cold sterilizing tray (US Dental Depot, 208GST‐2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA‐Advantage, 3‐948) Anesthesia induction chamber (SOMNI, IC‐3014) Oxygen concentrator (ProO 2 , Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non‐rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP‐1M) Temperature control unit (Kopf Instruments, TCAT‐2LV) Waste gas catch (SOMNI, WG‐15005) Dissecting microscope (Zeiss, Stemi 2000‐CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806‐WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High‐temperature, fine‐tip cautery (Bovie, AA01) Stainless steel screws (McMaster‐Carr, Phillips, no. 0, 1/8‐in. length, 92470A015) Syringe with 27‐G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/miniscope_v2.0/tree/main/STL‐miniscope ) and baseplate (Base_R007.SLDPRT, 3D‐Hubs) Microdrill: Cordless microdrill (KFTechnology) 0.1‐ and 1.0‐mm drill bits (Widget Supply, D‐DN01‐1pc and D‐DN12‐1pc) Standard electrode holder (Kopf Instruments, model 1770) GRIN lens probe: Standard electrode holder (Kopf Instruments, model 1770) Flexible PVC tubing (McMaster‐Carr, 5233K111) ..
Sterility:Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods.
Article Snippet: .. Materials Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019-360-60) O2 gas Eye ointment (Bausch & Lomb, 3-10119-02239-9) Dexamethasone (Bimeda, 1DEX022) 10% povidone-iodine antiseptic (Betadine, 67618-150-17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006-12) Graefe forceps (Fine Science Tools, 11053-10) Heavy blade dissector scissors (Fine Science Tools, 14082-09) Kelly hemostats (Fine Science Tools, 13019-14) Needle holder and scissors (Novo Surgical, G2171-25) Dissecting jewelers microforceps (Fisher Scientific, 08-953F) Periosteal elevators (Roboz, RS-8820) Surgical scalpel, #3 (Fine Science Tools, 10003-12) Cold sterilizing tray (US Dental Depot, 208GST-2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA-Advantage, 3-948) Anesthesia induction chamber (SOMNI, IC-3014) Oxygen concentrator (ProO2, Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non-rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP-1M) Temperature control unit (Kopf Instruments, TCAT-2LV) Waste gas catch (SOMNI, WG-15005) Dissecting microscope (Zeiss, Stemi 2000-CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806-WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High-temperature, fine-tip cautery (Bovie, AA01)Beacher et al. 4 of 21 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70090 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Stainless steel screws (McMaster-Carr, Phillips, no. 0, 1/8-in. length, 92470A015) Syringe with 27-G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/ miniscope_v2.0/ tree/main/STL-miniscope) and baseplate (Base_R007.
Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods
Article Snippet: .. Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019‐360‐60) O 2 gas Eye ointment (Bausch & Lomb, 3‐10119‐02239‐9) Dexamethasone (Bimeda, 1DEX022) 10% povidone‐iodine antiseptic (Betadine, 67618‐150‐17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006‐12) Graefe forceps (Fine Science Tools, 11053‐10) Heavy blade dissector scissors (Fine Science Tools, 14082‐09) Kelly hemostats (Fine Science Tools, 13019‐14) Needle holder and scissors (Novo Surgical, G2171‐25) Dissecting jewelers microforceps (Fisher Scientific, 08‐953F) Periosteal elevators (Roboz, RS‐8820) Surgical scalpel, #3 (Fine Science Tools, 10003‐12) Cold sterilizing tray (US Dental Depot, 208GST‐2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA‐Advantage, 3‐948) Anesthesia induction chamber (SOMNI, IC‐3014) Oxygen concentrator (ProO 2 , Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non‐rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP‐1M) Temperature control unit (Kopf Instruments, TCAT‐2LV) Waste gas catch (SOMNI, WG‐15005) Dissecting microscope (Zeiss, Stemi 2000‐CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806‐WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High‐temperature, fine‐tip cautery (Bovie, AA01) Stainless steel screws (McMaster‐Carr, Phillips, no. 0, 1/8‐in. length, 92470A015) Syringe with 27‐G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/miniscope_v2.0/tree/main/STL‐miniscope ) and baseplate (Base_R007.SLDPRT, 3D‐Hubs) Microdrill: Cordless microdrill (KFTechnology) 0.1‐ and 1.0‐mm drill bits (Widget Supply, D‐DN01‐1pc and D‐DN12‐1pc) Standard electrode holder (Kopf Instruments, model 1770) GRIN lens probe: Standard electrode holder (Kopf Instruments, model 1770) Flexible PVC tubing (McMaster‐Carr, 5233K111) ..
Control:Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods.
Article Snippet: .. Materials Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019-360-60) O2 gas Eye ointment (Bausch & Lomb, 3-10119-02239-9) Dexamethasone (Bimeda, 1DEX022) 10% povidone-iodine antiseptic (Betadine, 67618-150-17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006-12) Graefe forceps (Fine Science Tools, 11053-10) Heavy blade dissector scissors (Fine Science Tools, 14082-09) Kelly hemostats (Fine Science Tools, 13019-14) Needle holder and scissors (Novo Surgical, G2171-25) Dissecting jewelers microforceps (Fisher Scientific, 08-953F) Periosteal elevators (Roboz, RS-8820) Surgical scalpel, #3 (Fine Science Tools, 10003-12) Cold sterilizing tray (US Dental Depot, 208GST-2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA-Advantage, 3-948) Anesthesia induction chamber (SOMNI, IC-3014) Oxygen concentrator (ProO2, Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non-rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP-1M) Temperature control unit (Kopf Instruments, TCAT-2LV) Waste gas catch (SOMNI, WG-15005) Dissecting microscope (Zeiss, Stemi 2000-CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806-WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High-temperature, fine-tip cautery (Bovie, AA01)Beacher et al. 4 of 21 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70090 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Stainless steel screws (McMaster-Carr, Phillips, no. 0, 1/8-in. length, 92470A015) Syringe with 27-G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/ miniscope_v2.0/ tree/main/STL-miniscope) and baseplate (Base_R007.
Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods
Article Snippet: .. Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019‐360‐60) O 2 gas Eye ointment (Bausch & Lomb, 3‐10119‐02239‐9) Dexamethasone (Bimeda, 1DEX022) 10% povidone‐iodine antiseptic (Betadine, 67618‐150‐17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006‐12) Graefe forceps (Fine Science Tools, 11053‐10) Heavy blade dissector scissors (Fine Science Tools, 14082‐09) Kelly hemostats (Fine Science Tools, 13019‐14) Needle holder and scissors (Novo Surgical, G2171‐25) Dissecting jewelers microforceps (Fisher Scientific, 08‐953F) Periosteal elevators (Roboz, RS‐8820) Surgical scalpel, #3 (Fine Science Tools, 10003‐12) Cold sterilizing tray (US Dental Depot, 208GST‐2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA‐Advantage, 3‐948) Anesthesia induction chamber (SOMNI, IC‐3014) Oxygen concentrator (ProO 2 , Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non‐rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP‐1M) Temperature control unit (Kopf Instruments, TCAT‐2LV) Waste gas catch (SOMNI, WG‐15005) Dissecting microscope (Zeiss, Stemi 2000‐CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806‐WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High‐temperature, fine‐tip cautery (Bovie, AA01) Stainless steel screws (McMaster‐Carr, Phillips, no. 0, 1/8‐in. length, 92470A015) Syringe with 27‐G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/miniscope_v2.0/tree/main/STL‐miniscope ) and baseplate (Base_R007.SLDPRT, 3D‐Hubs) Microdrill: Cordless microdrill (KFTechnology) 0.1‐ and 1.0‐mm drill bits (Widget Supply, D‐DN01‐1pc and D‐DN12‐1pc) Standard electrode holder (Kopf Instruments, model 1770) GRIN lens probe: Standard electrode holder (Kopf Instruments, model 1770) Flexible PVC tubing (McMaster‐Carr, 5233K111) ..
Microscopy:Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods.
Article Snippet: .. Materials Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019-360-60) O2 gas Eye ointment (Bausch & Lomb, 3-10119-02239-9) Dexamethasone (Bimeda, 1DEX022) 10% povidone-iodine antiseptic (Betadine, 67618-150-17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006-12) Graefe forceps (Fine Science Tools, 11053-10) Heavy blade dissector scissors (Fine Science Tools, 14082-09) Kelly hemostats (Fine Science Tools, 13019-14) Needle holder and scissors (Novo Surgical, G2171-25) Dissecting jewelers microforceps (Fisher Scientific, 08-953F) Periosteal elevators (Roboz, RS-8820) Surgical scalpel, #3 (Fine Science Tools, 10003-12) Cold sterilizing tray (US Dental Depot, 208GST-2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA-Advantage, 3-948) Anesthesia induction chamber (SOMNI, IC-3014) Oxygen concentrator (ProO2, Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non-rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP-1M) Temperature control unit (Kopf Instruments, TCAT-2LV) Waste gas catch (SOMNI, WG-15005) Dissecting microscope (Zeiss, Stemi 2000-CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806-WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High-temperature, fine-tip cautery (Bovie, AA01)Beacher et al. 4 of 21 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70090 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Stainless steel screws (McMaster-Carr, Phillips, no. 0, 1/8-in. length, 92470A015) Syringe with 27-G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/ miniscope_v2.0/ tree/main/STL-miniscope) and baseplate (Base_R007.
Article Title: Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods
Article Snippet: .. Cidex disinfecting solution (ASP, CIDEX OPA, 20390) Rats of interest Isoflurane (Baxter, 10019‐360‐60) O 2 gas Eye ointment (Bausch & Lomb, 3‐10119‐02239‐9) Dexamethasone (Bimeda, 1DEX022) 10% povidone‐iodine antiseptic (Betadine, 67618‐150‐17) 70% (v/v) ethanol (NIH Supply Center) Surgical tools: Adson forceps (Fine Science Tools, 11006‐12) Graefe forceps (Fine Science Tools, 11053‐10) Heavy blade dissector scissors (Fine Science Tools, 14082‐09) Kelly hemostats (Fine Science Tools, 13019‐14) Needle holder and scissors (Novo Surgical, G2171‐25) Dissecting jewelers microforceps (Fisher Scientific, 08‐953F) Periosteal elevators (Roboz, RS‐8820) Surgical scalpel, #3 (Fine Science Tools, 10003‐12) Cold sterilizing tray (US Dental Depot, 208GST‐2) Surgical drape (Dynarex, 4410) Sterile instrument tray (GSA‐Advantage, 3‐948) Anesthesia induction chamber (SOMNI, IC‐3014) Oxygen concentrator (ProO 2 , Pro 5 625) Isoflurane vaporizer (SOMNI, Tec 5 Funnel Fill) Electric Clippers (Philips Norelco, QT4070/41) Stereotaxic apparatus and attachments: Small animal stereotaxic instrument (Kopf Instruments, model 962) Non‐rupture rat ear bars (Kopf Instruments, model 955) Rat anesthesia mask (Kopf Instruments, model 906) Heating pad wrapped in plastic sleeve (Kopf Instruments, HP‐1M) Temperature control unit (Kopf Instruments, TCAT‐2LV) Waste gas catch (SOMNI, WG‐15005) Dissecting microscope (Zeiss, Stemi 2000‐CS) Microscope stand (Omano, V7 Articulating Boom Stand) Cotton tipped applicators (Puritan, 806‐WC) Scalpel blade, #10 (Integra Miltex 4110) Gauze sponges (Covidien Curity, 2556) High‐temperature, fine‐tip cautery (Bovie, AA01) Stainless steel screws (McMaster‐Carr, Phillips, no. 0, 1/8‐in. length, 92470A015) Syringe with 27‐G needle Baseplate assembly (Giovanni Barbera, https://github.com/giovannibarbera/miniscope_v2.0/tree/main/STL‐miniscope ) and baseplate (Base_R007.SLDPRT, 3D‐Hubs) Microdrill: Cordless microdrill (KFTechnology) 0.1‐ and 1.0‐mm drill bits (Widget Supply, D‐DN01‐1pc and D‐DN12‐1pc) Standard electrode holder (Kopf Instruments, model 1770) GRIN lens probe: Standard electrode holder (Kopf Instruments, model 1770) Flexible PVC tubing (McMaster‐Carr, 5233K111) ..
|
